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Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

DOI:
10.3791/57369-v

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09:06 min

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May 20, 2018

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, , , ,
1Omics Core Lab, Research Institute,National Cancer Center, 2Department of Pathology,Inje University Ilsan Paik Hospital

Chapters

  • 00:04Title
  • 00:40HeLa Cell Serum Starvation
  • 01:49Cell Block Preparation
  • 03:01Tissue Processing and Paraffin Embedding
  • 03:50Immunocytochemical Clot Analysis
  • 06:50Results: Representative Histological and Immunocytochemical Analyses
  • 07:58Conclusion

Summary

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Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.

Tags

ImmunocytochemistryParaffin-embedded Cell BlocksProtein ExpressionProliferation MarkersHeLa CellsEDTA-trypsinPlasmaThromboplastinCalcium ChlorideFormalin Fixation

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