Journal
/
癌症研究
/
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
JoVE 杂志
癌症研究
This content is Free Access.
JoVE 杂志 癌症研究
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

11,094 Views

•

09:06 min

•

May 20, 2018

DOI:

10.3791/57369-v

DOI:
10.3791/57369-v

•

09:06 min
May 20, 2018

•

7 Views
, , , ,
1Omics Core Lab, Research Institute,National Cancer Center, 2Department of Pathology,Inje University Ilsan Paik Hospital

Summary

Automatically generated

Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.

Explore More Videos

ImmunocytochemistryParaffin-embedded Cell BlocksProtein ExpressionProliferation MarkersHeLa CellsEDTA-trypsinPlasmaThromboplastinCalcium ChlorideFormalin Fixation

Read Article

Cite this Article

Poojan, S., Kim, H., Yoon, J., Sim, H. W., Hong, K. Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks. J. Vis. Exp. (135), e57369, doi:10.3791/57369 (2018).

Download .ris file

Copy

Share Video

.

Copy