This content is Free Access.
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
章
- 00:04標題
- 00:40HeLa Cell Serum Starvation
- 01:49Cell Block Preparation
- 03:01Tissue Processing and Paraffin Embedding
- 03:50Immunocytochemical Clot Analysis
- 06:50Results: Representative Histological and Immunocytochemical Analyses
- 07:58Conclusion
Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.
