Journal
/
Krebsforschung
/
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
JoVE Journal
Krebsforschung
This content is Free Access.
JoVE Journal Krebsforschung
Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

DOI:
10.3791/57369-v

•

09:06 min

•

May 20, 2018

•

, , , ,
1Omics Core Lab, Research Institute,National Cancer Center, 2Department of Pathology,Inje University Ilsan Paik Hospital

Kapitel

  • 00:04Titel
  • 00:40HeLa Cell Serum Starvation
  • 01:49Cell Block Preparation
  • 03:01Tissue Processing and Paraffin Embedding
  • 03:50Immunocytochemical Clot Analysis
  • 06:50Results: Representative Histological and Immunocytochemical Analyses
  • 07:58Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Currently, immunofluorescent staining on fixed cells is the method of choice for determination of protein expression levels when morphological information is also necessary. This protocol presented herein provides for an alternative method of immunocytochemistry on paraffin-embedded cell blocks.

Tags

ImmunocytochemistryParaffin-embedded Cell BlocksProtein ExpressionProliferation MarkersHeLa CellsEDTA-trypsinPlasmaThromboplastinCalcium ChlorideFormalin Fixation

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

Read Article