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具有量身定做多样性的合成噬菌体展示库的构建
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
DOI:

12:31 min

May 01, 2018

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Chapters

  • 00:04Title
  • 01:18Electroporation and Calculation of the Library Size
  • 06:02Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
  • 09:43Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
  • 11:07Conclusion

Summary

Automatic Translation

本议定书描述了一个详细的程序, 以构建一个噬菌体显示合成抗体库, 具有量身定做的多样性。合成抗体具有广泛的应用, 从基础研究到疾病诊断和治疗。

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