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合成のバクテリオファージの建設は合わせた多様性と fab 抗体ライブラリーを表示
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Immunology and Infection
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JoVE Journal Immunology and Infection
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Please note that all translations are automatically generated. Click here for the English version.

合成のバクテリオファージの建設は合わせた多様性と fab 抗体ライブラリーを表示

日本語

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13,221 Views

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12:31 min

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May 01, 2018

DOI:

10.3791/57357-v

DOI:
10.3791/57357-v

•

12:31 min
May 01, 2018

•

21 Views
, , , , ,
1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto

Summary

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このプロトコルでは、合わせた多様性を持つ合成抗体のバクテリオファージの表示ライブラリの構築の詳細な手順について説明します。合成抗体は、病気の診断、治療に基礎研究から広範なアプリケーションを持っています。

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Cite this Article

Huang, G., Zhong, Z., Miersch, S., Sidhu, S. S., Hou, S., Wu, D. Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity. J. Vis. Exp. (135), e57357, doi:10.3791/57357 (2018).

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