This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and therapeutics.
Huang, G., Zhong, Z., Miersch, S., Sidhu, S. S., Hou, S., Wu, D. Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity. J. Vis. Exp. (135), e57357, doi:10.3791/57357 (2018).