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Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
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May 01, 2018
DOI:
1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto
Summary
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This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and therapeutics.
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Huang, G., Zhong, Z., Miersch, S., Sidhu, S. S., Hou, S., Wu, D. Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity. J. Vis. Exp. (135), e57357, doi:10.3791/57357 (2018).
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