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Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
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Immunology and Infection
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JoVE Journal Immunology and Infection
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

DOI:
10.3791/57357-v

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12:31 min

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May 01, 2018

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, , , , ,
1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto

Chapters

  • 00:04Title
  • 01:18Electroporation and Calculation of the Library Size
  • 06:02Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
  • 09:43Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
  • 11:07Conclusion

Summary

Automatic Translation

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This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and therapeutics.

Tags

Synthetic Phage DisplayFab LibraryAntibody Library ConstructionAntibody DiversityMonoclonal Antibody DevelopmentPhage Display TechnologyKunkel MutagenesisElectroporationDiversity EvaluationELISA

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