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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
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発生生物学
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JoVE Journal 発生生物学
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

19,992 Views

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07:18 min

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April 19, 2018

DOI:

10.3791/57212-v

DOI:
10.3791/57212-v

•

07:18 min
April 19, 2018

•

31 Views
, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health (NIH), 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health (NIH)

概要

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The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.

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Skeletal MuscleSatellite CellsImmunofluorescencePax7LamininCryosectioningCryostatAntigen RetrievalImmunostaining

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Cite this Article

Feng, X., Naz, F., Juan, A. H., Dell'Orso, S., Sartorelli, V. Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies. J. Vis. Exp. (134), e57212, doi:10.3791/57212 (2018).

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