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牙龈三维炎症人体组织当量
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
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牙龈三维炎症人体组织当量

DOI:
10.3791/57157-v

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08:43 min

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April 03, 2018

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, ,
1Department of Pharmacology,The Nippon Dental University, 2Department of Oral and Maxillofacial Surgery,The Nippon Dental University Hospital

Chapters

  • 00:04Title
  • 00:46Preparation of 3D Human Tissue Equivalents of Gingiva
  • 04:17Preparation of Wounded and Inflammatory GTE
  • 05:46Results: Characterize the 3D Inflammatory Human Tissue Equivalents of Gingiva (iGTE)
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目标是建立一个炎症的人牙龈模型在体外。该组织模型共培养三种类型的人细胞, HaCaT 角质形成细胞, 牙龈成纤维细胞, THP-1 巨噬细胞, 在三维条件下。该模型可用于牙周疾病的研究, 如牙龈炎和牙周炎。

Tags

3DInflammatoryGingivaIn VitroHuman CellsWound HealingInflammationPeriodontal DiseasesCollagenHGFHaCaTCulture InsertCulture MediumCell SuspensionCo-culture

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