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脂肪酸¹³C Isotopologue 分析提供了对无脊椎动物消费者营养碳转移和脂质代谢的洞察力

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Biochemistry

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JoVE Journal Biochemistry

Fatty Acid ¹³C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Please note that all translations are automatically generated. Click here for the English version.

脂肪酸¹³C Isotopologue 分析提供了对无脊椎动物消费者营养碳转移和脂质代谢的洞察力

DOI:

10.3791/57110-v

•

11:14 min

•

April 17, 2018

•

Ralph Menzel, Rainer Nehring, Dilara Simsek, Liliane Ruess

¹Ecology, Institute of Biology,Humboldt-Universität zu Berlin

Chapters

00:04Title
00:45Cultivation of Animals
03:08Lipid Extraction from Animal Tissue and Methanolysis
05:19Quantification of Fatty Acids by GC-FID and ¹³C Analysis by Isotopologue Profiling
08:29Results: Fatty Acid ¹³C Isotopologue Profiling in Collembola
10:32Conclusion

Summary

Automatic Translation

脂肪酸营养标记方法,即,将脂肪酸同化为整个分子, 并转移到没有或轻微修饰的消费者组织中, 这是由于小土壤无脊椎动物脂肪酸代谢的知识缺口所阻碍的。Isotopologue 分析作为一种宝贵的工具来解开营养的相互作用。

Tags

Fatty Acid 13C Isotopologue Trophic Transfer Lipid Metabolism Invertebrate Consumers Food Web Ecology Collembola Plaster Microcosms Lipid Extraction GCMS Analysis

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