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Journal / Biochemistry / Fatty Acid 13C Isotopologue Profiling Provides…
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Biochemistry

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JoVE Journal Biochemistry
Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
 

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Article DOI: 10.3791/57110-v • 11:14 min • April 17th, 2018
April 17th, 2018 •
1, 1, 1, 1
1Ecology, Institute of Biology, Humboldt-Universität zu Berlin

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The fatty acid trophic marker approach, i.e., the assimilation of fatty acids as entire molecule and transfer into consumer tissue with no or minor modification, is hampered by knowledge gaps in fatty acid metabolism of small soil invertebrates. Isotopologue profiling is provided as a valuable tool to disentangle trophic interactions.

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Keywords: Fatty Acid 13C Isotopologue Trophic Transfer Lipid Metabolism Invertebrate Consumers Food Web Ecology Collembola Plaster Microcosms Lipid Extraction GCMS Analysis
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