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Hemocytes mikrobiyal enfeksiyon ve çözümleme çekme--dan Drosophila melanogaster larva
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Immunology and Infection
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JoVE Journal Immunology and Infection
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
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Hemocytes mikrobiyal enfeksiyon ve çözümleme çekme--dan Drosophila melanogaster larva

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11,344 Views

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08:54 min

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May 24, 2018

DOI:

10.3791/57077-v

DOI:
10.3791/57077-v

•

08:54 min
May 24, 2018

•

15 Views
, ,
1School of Molecular Biosciences, College of Veterinary Medicine,Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine,Washington State University

Summary

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Bu yöntem, patojen işgali üç boyutlu (3D) modelleri ile böcek hücreye görselleştirmek gösterilmiştir. Hemocytes Drosophila larvaları üzerinden viral veya bakteriyel patojenler, ex vivo veya vivo içindeile bulaşmış. Virüslü hemocytes daha sonra sabit ve görüntüleme için bir confocal mikroskop ve sonraki 3D hücresel yeniden yapılanma ile lekeli.

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Hiroyasu, A., DeWitt, D. C., Goodman, A. G. Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis. J. Vis. Exp. (135), e57077, doi:10.3791/57077 (2018).

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