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Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

English

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11,344 Views

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08:54 min

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May 24, 2018

DOI:

10.3791/57077-v

DOI:
10.3791/57077-v

•

08:54 min
May 24, 2018

•

15 Views
, ,
1School of Molecular Biosciences, College of Veterinary Medicine,Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine,Washington State University

Summary

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This method demonstrates how to visualize pathogen invasion into insect cells with three-dimensional (3D) models. Hemocytes from Drosophila larvae were infected with viral or bacterial pathogens, either ex vivo or in vivo. Infected hemocytes were then fixed and stained for imaging with a confocal microscope and subsequent 3D cellular reconstruction.

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Hiroyasu, A., DeWitt, D. C., Goodman, A. G. Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis. J. Vis. Exp. (135), e57077, doi:10.3791/57077 (2018).

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