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Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

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08:54 min

May 24, 2018

DOI:

08:54 min
May 24, 2018

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Summary

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This method demonstrates how to visualize pathogen invasion into insect cells with three-dimensional (3D) models. Hemocytes from Drosophila larvae were infected with viral or bacterial pathogens, either ex vivo or in vivo. Infected hemocytes were then fixed and stained for imaging with a confocal microscope and subsequent 3D cellular reconstruction.

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