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Winning van Hemocytes van Drosophila melanogaster larven voor microbiële infectie en analyse
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Immunology and Infection
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JoVE Journal Immunology and Infection
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
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Winning van Hemocytes van Drosophila melanogaster larven voor microbiële infectie en analyse

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11,344 Views

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08:54 min

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May 24, 2018

DOI:

10.3791/57077-v

DOI:
10.3791/57077-v

•

08:54 min
May 24, 2018

•

15 Views
, ,
1School of Molecular Biosciences, College of Veterinary Medicine,Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine,Washington State University

Summary

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Deze methode laat zien hoe om te visualiseren pathogen invasie in insect cellen met driedimensionale (3D) modellen. Hemocytes van Drosophila larven waren besmet met virale of bacteriële pathogenen, ex vivo of in vivo. Besmette hemocytes werden vervolgens vast en gekleurd voor imaging met een confocal microscoop en de daaropvolgende 3D cellulaire wederopbouw.

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Hiroyasu, A., DeWitt, D. C., Goodman, A. G. Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis. J. Vis. Exp. (135), e57077, doi:10.3791/57077 (2018).

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