A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:39Preparation of Zebrafish Specimen
- 02:12Microscope Mounting and Pre-conversion Imaging
- 03:35Single-cell Photoconversion
- 06:18Post-photoconversion Imaging
- 07:38Results: Photoconverted Eos Protein in Single and a Population of Ganglion Cells in Transgenic Zebrafish
- 08:53Conclusion
Tags
Single-cell PhotoconversionZebrafishFluorescent Cell PopulationsDevelopmental QuestionsSingle-cell MigrationMinimally InvasivePhotoconvertible Protein488 Nanometer Light SourceGFP Filter SetsDechorionateLow Melting Point AgaroseAnesthetized Transgenic Sox10 Eos Positive FishConfocal MicroscopyPre-conversion ImagingDorsal Root GanglionC488 Laser3D Capture
