È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Single-cell Photoconversion in Living Intact Zebrafish
5,856 Views
•
09:49 min
•
March 19, 2018
DOI:
Summary
Automatically generated
Here, we present a protocol to show how cell photoconversion is achieved through UV exposure to specific areas expressing the fluorescent protein, Eos, in living animals.
Explore More Videos
Single-cell PhotoconversionZebrafishFluorescent Cell PopulationsDevelopmental QuestionsSingle-cell MigrationMinimally InvasivePhotoconvertible Protein488 Nanometer Light SourceGFP Filter SetsDechorionateLow Melting Point AgaroseAnesthetized Transgenic Sox10 Eos Positive FishConfocal MicroscopyPre-conversion ImagingDorsal Root GanglionC488 Laser3D Capture
Read Article
Cite this Article
Green, L., Smith, C. J. Single-cell Photoconversion in Living Intact Zebrafish. J. Vis. Exp. (133), e57024, doi:10.3791/57024 (2018).
Copy