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Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo
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Developmental Biology
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JoVE Journal Developmental Biology
Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

English

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6,368 Views

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09:38 min

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February 20, 2018

DOI:

10.3791/57290-v

DOI:
10.3791/57290-v

•

09:38 min
February 20, 2018

•

4 Views
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1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2UMR7104,Centre National de la Recherche Scientifique, 3U964,Institut National de la Santé et de la Recherche Médicale, 4Université de Strasbourg

Summary

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This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development.

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Cite this Article

Chow, R. W., Lamperti, P., Steed, E., Boselli, F., Vermot, J. Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo. J. Vis. Exp. (132), e57290, doi:10.3791/57290 (2018).

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