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Lökosit işe kültürlü damar hücreleri ve yapışık trombositler üzerinde araştırmak için Laminar akış tabanlı deneyleri
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Immunology and Infection
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JoVE Journal Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
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Lökosit işe kültürlü damar hücreleri ve yapışık trombositler üzerinde araştırmak için Laminar akış tabanlı deneyleri

Türkçe

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7,436 Views

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08:50 min

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April 09, 2018

DOI:

10.3791/57009-v

DOI:
10.3791/57009-v

•

08:50 min
April 09, 2018

•

6 Views
, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University, School of Medicine

Summary

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Lökosit heyecanla damar hücreleri ve trombositlerin damar duvar yaralanma sonra veya enflamasyon sırasında etkileşim. Burada, lökosit ve hücresel eşleri arasındaki etkileşimler altında yatan moleküler mekanizmaları karakterize etmek için basit bir laminar akış tabanlı tahlil açıklayın.

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Keyword Extraction:Leukocyte RecruitmentAdherent Vascular CellsAdherent PlateletsFlow ConditionsLaminar Flow-based AssaysImmunologyLeukocyte AdhesionTumor Cell AdhesionPlatelet-rich PlasmaPlatelet-poor PlasmaPlatelet BufferHydrochloric Acid EthanolRat Collagen Type OnePhosphate Buffered Saline

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Cite this Article

Vajen, T., Heinzmann, A. C., Dickhout, A., Zhao, Z., Nagy, M., Heemskerk, J. W., Koenen, R. R. Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets. J. Vis. Exp. (134), e57009, doi:10.3791/57009 (2018).

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