Dergi
/
İmmünoloji ve Enfeksiyon
/
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

DOI:
10.3791/57009-v

•

08:50 min

•

April 09, 2018

•

, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University, School of Medicine

Bölümler

  • 00:05Başlık
  • 00:57Flow-based Assay with Human Cells
  • 02:28Preparation of Adherent Platelet and Vascular Cell Monolayers
  • 03:56Fluorescent Labeling and Perfusion of Leukocytes
  • 06:32Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
  • 08:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Leukocytes avidly interact with vascular cells and platelets after vessel wall injury or during inflammation. Here, we describe a straightforward laminar flow-based assay to characterize the molecular mechanisms that underlie the interactions between leukocytes and their cellular partners.

Etiketler

Keyword Extraction:Leukocyte RecruitmentAdherent Vascular CellsAdherent PlateletsFlow ConditionsLaminar Flow-based AssaysİmmünolojiLeukocyte AdhesionTumor Cell AdhesionPlatelet-rich PlasmaPlatelet-poor PlasmaPlatelet BufferHydrochloric Acid EthanolRat Collagen Type OnePhosphate Buffered Saline

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Read Article