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Ensaios baseados em fluxo laminar para investigar o recrutamento de leucócitos em culturas de células vasculares e plaquetas aderentes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Ensaios baseados em fluxo laminar para investigar o recrutamento de leucócitos em culturas de células vasculares e plaquetas aderentes

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7,436 Views

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08:50 min

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April 09, 2018

DOI:

10.3791/57009-v

DOI:
10.3791/57009-v

•

08:50 min
April 09, 2018

•

6 Views
, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University, School of Medicine

Summary

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Leucócitos, avidamente, interagir com células vasculares e plaquetas após lesão de parede do vaso ou durante a inflamação. Aqui, descrevemos um simples ensaio baseado em fluxo laminar para caracterizar os mecanismos moleculares que fundamentam as interações entre os leucócitos e os seus parceiros de celulares.

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Keyword Extraction:Leukocyte RecruitmentAdherent Vascular CellsAdherent PlateletsFlow ConditionsLaminar Flow-based AssaysImmunologyLeukocyte AdhesionTumor Cell AdhesionPlatelet-rich PlasmaPlatelet-poor PlasmaPlatelet BufferHydrochloric Acid EthanolRat Collagen Type OnePhosphate Buffered Saline

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Vajen, T., Heinzmann, A. C., Dickhout, A., Zhao, Z., Nagy, M., Heemskerk, J. W., Koenen, R. R. Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets. J. Vis. Exp. (134), e57009, doi:10.3791/57009 (2018).

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