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Immunology and Infection

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JoVE Journal Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
 

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Article DOI: 10.3791/57009-v • 08:50 min • April 9th, 2018
April 9th, 2018 •
1, 1, 1, 2, 1, 1, 1
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine

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Summary
Automatic Translation

Leukocytes avidly interact with vascular cells and platelets after vessel wall injury or during inflammation. Here, we describe a straightforward laminar flow-based assay to characterize the molecular mechanisms that underlie the interactions between leukocytes and their cellular partners.

Tags

Keyword Extraction: Leukocyte Recruitment Adherent Vascular Cells Adherent Platelets Flow Conditions Laminar Flow-based Assays Immunology Leukocyte Adhesion Tumor Cell Adhesion Platelet-rich Plasma Platelet-poor Plasma Platelet Buffer Hydrochloric Acid Ethanol Rat Collagen Type One Phosphate Buffered Saline
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