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活蛙 (爪蟾) 胚胎单细胞代谢的微探针毛细管电泳质谱
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JoVE Journal Chemistry
Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos
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活蛙 (爪蟾) 胚胎单细胞代谢的微探针毛细管电泳质谱

DOI:
10.3791/56956-v

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12:16 min

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December 22, 2017

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, , ,
1Department of Chemistry,George Washington University, 2Department of Anatomy & Regenerative Biology,George Washington University, 3Department of Chemistry & Biochemistry,University of Maryland, College Park

Chapters

  • 00:05Title
  • 01:01Preparation of Sampling Instruments and Dishes
  • 02:01Microsampling Single Cells and Metabolite Extraction
  • 05:23Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS) Measurement
  • 09:01Results: Metabolic Differences Between Single Cells in the Early Developing Xenopus laevis Embryo
  • 10:43Conclusion

Summary

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我们描述的步骤, 使快速的原位取样的一个小部分, 高精度和最小的入侵使用 capillary-based 微取样, 以促进化学表征的代谢活动的快照活胚胎使用 custom-built 单细胞毛细管电泳和质谱仪平台。

Tags

MicroprobeCapillary ElectrophoresisMass SpectrometrySingle-cell MetabolomicsLive FrogXenopus LaevisEmbryosIn Situ MicrosamplingCell BiologyDevelopmental BiologyMinimally Invasive SamplingAgarose GelPasteur PipetteBorosilicate CapillariesFlaming/Brown Type Capillary PullerDejellyingSteinberg’s SolutionGonadotropinIn Vitro Fertilization

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