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Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
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Chemistry
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JoVE Journal Chemistry
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

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9,714 Views

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10:13 min

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November 12, 2016

DOI:

10.3791/54611-v

DOI:
10.3791/54611-v

•

10:13 min
November 12, 2016

•

5 Views
, ,
1Center for Applied Plant Sciences,The Ohio State University, OARDC, 2Department of Plant Pathology,The Ohio State University, OARDC, 3Department of Horticulture and Crop Science,The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster,The Ohio State University, OARDC

Summary

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This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.

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Cite this Article

Vaughan, M. J., Chanon, A., Blakeslee, J. J. Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds. J. Vis. Exp. (117), e54611, doi:10.3791/54611 (2016).

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