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Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos
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Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

19,206 Views

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12:16 min

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December 22, 2017

DOI:

10.3791/56956-v

DOI:
10.3791/56956-v

•

12:16 min
December 22, 2017

•

2 Views
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1Department of Chemistry,George Washington University, 2Department of Anatomy & Regenerative Biology,George Washington University, 3Department of Chemistry & Biochemistry,University of Maryland, College Park

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MicroprobeCapillary ElectrophoresisMass SpectrometrySingle-cell MetabolomicsLive FrogXenopus LaevisEmbryosIn Situ MicrosamplingCell BiologyEntwicklungsbiologieMinimally Invasive SamplingAgarose GelPasteur PipetteBorosilicate CapillariesFlaming/Brown Type Capillary PullerDejellyingSteinberg’s SolutionGonadotropinIn Vitro Fertilization

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Onjiko, R. M., Portero, E. P., Moody, S. A., Nemes, P. Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos. J. Vis. Exp. (130), e56956, doi:10.3791/56956 (2017).

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