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Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos
 

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Article DOI: 10.3791/56956-v • 12:16 min • December 22nd, 2017
December 22nd, 2017 •
1, 1, 2, 1,3
1Department of Chemistry, George Washington University, 2Department of Anatomy & Regenerative Biology, George Washington University, 3Department of Chemistry & Biochemistry, University of Maryland, College Park

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We describe steps that enable fast in situ sampling of a small portion of an individual cell with high precision and minimal invasion using capillary-based micro-sampling, to facilitate chemical characterization of a snapshot of metabolic activity in live embryos using a custom-built single cell capillary electrophoresis and mass spectrometry platform.

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Keywords: Microprobe Capillary Electrophoresis Mass Spectrometry Single-cell Metabolomics Live Frog Xenopus Laevis Embryos In Situ Microsampling Cell Biology Developmental Biology Minimally Invasive Sampling Agarose Gel Pasteur Pipette Borosilicate Capillaries Flaming/Brown Type Capillary Puller Dejellying Steinberg's Solution Gonadotropin In Vitro Fertilization
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