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Deteção de fosforilação Rab10 pela atividade LRRK2 utilizando SDS-PAGE com um Tag de ligação de fosfato
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Biochemistry
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JoVE Journal Biochemistry
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Deteção de fosforilação Rab10 pela atividade LRRK2 utilizando SDS-PAGE com um Tag de ligação de fosfato

português

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14,331 Views

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08:55 min

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December 14, 2017

DOI:

10.3791/56688-v

DOI:
10.3791/56688-v

•

08:55 min
December 14, 2017

•

13 Views
,
1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Summary

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O presente estudo descreve um método simples de detectar níveis endógenos de fosforilação de Rab10 pela rica em leucina repetição quinase 2.

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Cite this Article

Ito, G., Tomita, T. Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag. J. Vis. Exp. (130), e56688, doi:10.3791/56688 (2017).

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