Dergi
/
Biyokimya
/
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

14,269 Views

•

08:55 min

•

December 14, 2017

DOI:

10.3791/56688-v

DOI:
10.3791/56688-v

•

08:55 min
December 14, 2017

•

13 Views
,
1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Özet

Automatically generated

The present study describes a simple method of detecting endogenous levels of Rab10 phosphorylation by leucine-rich repeat kinase 2.

Explore More Videos

Rab10 PhosphorylationLRRK2 ActivitySDS-PAGEPhosphate-binding TagParkinson’s ResearchHEK293 CellsCell LysisBradford AssaySample PreparationSDS-PAGE GelManganese Chloride

Read Article

Cite this Article

Ito, G., Tomita, T. Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag. J. Vis. Exp. (130), e56688, doi:10.3791/56688 (2017).

Download .ris file

Copy

Share Video

.

Copy