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SDS ページを用いたリン酸 binding タグ LRRK2 活動によって Rab10 リン酸化検出
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Biochemistry
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JoVE Journal Biochemistry
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
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SDS ページを用いたリン酸 binding タグ LRRK2 活動によって Rab10 リン酸化検出

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14,331 Views

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08:55 min

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December 14, 2017

DOI:

10.3791/56688-v

DOI:
10.3791/56688-v

•

08:55 min
December 14, 2017

•

13 Views
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1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Summary

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本研究では、ロイシン豊富な繰り返しキナーゼ 2 によって Rab10 リン酸化の内因性のレベルを検出する簡単な方法について説明します。

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Cite this Article

Ito, G., Tomita, T. Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag. J. Vis. Exp. (130), e56688, doi:10.3791/56688 (2017).

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