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Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
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Biochemistry
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JoVE Journal Biochemistry
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

DOI:
10.3791/56688-v

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08:55 min

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December 14, 2017

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1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Chapters

  • 00:05Title
  • 00:53Collecting Cell Lysates
  • 02:34SDS-PAGE and Gel Transfer
  • 04:41Immunoblotting
  • 06:22Results: P-tag Gel Analysis
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

The present study describes a simple method of detecting endogenous levels of Rab10 phosphorylation by leucine-rich repeat kinase 2.

Tags

Rab10 PhosphorylationLRRK2 ActivitySDS-PAGEPhosphate-binding TagParkinson’s ResearchHEK293 CellsCell LysisBradford AssaySample PreparationSDS-PAGE GelManganese Chloride

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