Journal
/
Biochemistry
/
用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Please note that all translations are automatically generated. Click here for the English version.

用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

English

Automatically Generated

14,133 Views

•

08:55 min

•

December 14, 2017

DOI:

10.3791/56688-v

DOI:
10.3791/56688-v

•

08:55 min
December 14, 2017

•

13 Views
,
1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Summary

Automatically generated

本研究介绍了一种简单的方法检测内源性水平的 Rab10 磷酸化富亮氨酸重复激酶2。

Explore More Videos

Rab10 PhosphorylationLRRK2 ActivitySDS-PAGEPhosphate-binding TagParkinson’s ResearchHEK293 CellsCell LysisBradford AssaySample PreparationSDS-PAGE GelManganese Chloride

Read Article

Cite this Article

Ito, G., Tomita, T. Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag. J. Vis. Exp. (130), e56688, doi:10.3791/56688 (2017).

Download .ris file

Copy

Share Video

.

Copy