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На основе RANKL остеокластов культуры Assay из мыши костного расследовать роль mTORC1 в формировании остеокластов
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09:37 min
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March 15, 2018
DOI:
1Department of Pediatric Dentistry, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine
Summary
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Эта рукопись описывает протокол к изоляции и культуры остеокласты в пробирке из костного мозга мыши и для изучения роли млекопитающих/механистический цель rapamycin комплекс 1 в формировании остеокластов.
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Cite this Article
Dai, Q., Han, Y., Xie, F., Ma, X., Xu, Z., Liu, X., Zou, W., Wang, J. A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation. J. Vis. Exp. (133), e56468, doi:10.3791/56468 (2018).
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