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Une base RANKL Osteoclast Culture dosage de moelle osseuse de souris pour enquêter sur le rôle de mTORC1 dans la Formation des ostéoclastes
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09:37 min
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March 15, 2018
DOI:
1Department of Pediatric Dentistry, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine
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Ce manuscrit décrit un protocole d’isolat et la culture les ostéoclastes in vitro de la moelle osseuse de souris et d’étudier le rôle de la cible mammifère/mécaniste de la rapamycine complexe 1 dans la formation des ostéoclastes.
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Cite this Article
Dai, Q., Han, Y., Xie, F., Ma, X., Xu, Z., Liu, X., Zou, W., Wang, J. A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation. J. Vis. Exp. (133), e56468, doi:10.3791/56468 (2018).
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