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Ein RANKL-basierte Osteoklasten Kultur Assay der Maus Knochenmark zu untersuchen, die Rolle von mTORC1 in Osteoklasten Bildung
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09:37 min
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March 15, 2018
DOI:
1Department of Pediatric Dentistry, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine
Summary
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Dieses Manuskript beschreibt ein Protokoll zum isolieren und Kultur Osteoklasten in Vitro aus Maus Knochenmark und zur Untersuchung der Rolle von Säugetieren/mechanistischen Ziel von Rapamycin Komplex 1 in Osteoklasten-Formation.
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Cite this Article
Dai, Q., Han, Y., Xie, F., Ma, X., Xu, Z., Liu, X., Zou, W., Wang, J. A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation. J. Vis. Exp. (133), e56468, doi:10.3791/56468 (2018).
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