Journal
/
Entwicklungsbiologie
/
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

DOI:
10.3791/56468-v

•

09:37 min

•

March 15, 2018

•

, , , , , , ,
1Department of Pediatric Dentistry, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine

Kapitel

  • 00:05Titel
  • 00:59Dissection and Isolation
  • 03:27Plating and Differentiation
  • 05:41Tartrate Resistant Acid Phosphatase Staining and Activity Quantification of Culture Medium
  • 07:25Results: Deletion of Raptor Impaired Osteoclast Formation
  • 08:27Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This manuscript describes a protocol to isolate and culture osteoclasts in vitro from mouse bone marrow, and to study the role of the mammalian/mechanistic target of rapamycin complex 1 in osteoclast formation.

Tags

OsteoclastRANKLMTORC1Bone MarrowIn Vitro CultureOsteoporosisCell IsolationCell Differentiation

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

Read Article