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11:15 min
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March 02, 2018
DOI:
1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry,University of Washington
Summary
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Wir berichten über eine kleine Hairpin RNA (ShRNA) und die nächste Generation Sequencing-basiertes Protokoll zur Identifizierung von Regulatoren der X-Chromosom Inaktivierung in einem murinen Zelllinie mit Firefly Luciferase und Hygromycin-Resistenz-Gene verschmolzen, das Methyl CpG verbindliches Protein 2) MeCP2) gen auf dem x-Chromosom inaktiv.
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Cite this Article
Leko, V., Sripathy, S., Adrianse, R. L., Loe, T., Park, A., Lao, U., Foss, E. J., Bartolomei, M. S., Bedalov, A. Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome. J. Vis. Exp. (133), e56398, doi:10.3791/56398 (2018).
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