We report a small hairpin RNA (shRNA) and next generation sequencing-based protocol for identifying regulators of X-chromosome inactivation in a murine cell line with firefly luciferase and hygromycin resistance genes fused to the methyl CpG binding protein 2 (MeCP2) gene on the inactive X chromosome.
Leko, V., Sripathy, S., Adrianse, R. L., Loe, T., Park, A., Lao, U., Foss, E. J., Bartolomei, M. S., Bedalov, A. Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome. J. Vis. Exp. (133), e56398, doi:10.3791/56398 (2018).