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Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
JoVE Journal
Genetica
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JoVE Journal Genetica
Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

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11:15 min

March 02, 2018

DOI:

11:15 min
March 02, 2018

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Summary

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We report a small hairpin RNA (shRNA) and next generation sequencing-based protocol for identifying regulators of X-chromosome inactivation in a murine cell line with firefly luciferase and hygromycin resistance genes fused to the methyl CpG binding protein 2 (MeCP2) gene on the inactive X chromosome.

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