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G2-seq: Uma alta taxa de transferência baseada no sequenciamento técnica para identificar a tarde replicando regiões do genoma
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Genetics
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JoVE Journal Genetics
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: Uma alta taxa de transferência baseada no sequenciamento técnica para identificar a tarde replicando regiões do genoma

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5,630 Views

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06:40 min

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March 22, 2018

DOI:

10.3791/56286-v

DOI:
10.3791/56286-v

•

06:40 min
March 22, 2018

•

2 Views
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1Division of Clinical Research,Fred Hutchinson Cancer Research Center, 2Departments of Medicine and Biochemistry,University of Washington

Summary

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Nós descrevemos uma técnica de combinação de citometria de fluxo e sequenciamento de alto throughput para identificar regiões de replicação finais do genoma.

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G2-seqHigh-throughput SequencingDNA ReplicationCell CycleLate Replicating RegionsGenomeYeastFlow CytometryCell SortingDNA ContentG1SG2 Phases

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Foss, E. J., Lao, U., Bedalov, A. G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome. J. Vis. Exp. (133), e56286, doi:10.3791/56286 (2018).

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