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G2-seq: 一种基于高通量测序技术的基因组晚期复制区域识别方法
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Genetics
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JoVE Journal Genetics
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: 一种基于高通量测序技术的基因组晚期复制区域识别方法

DOI:
10.3791/56286-v

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06:40 min

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March 22, 2018

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, ,
1Division of Clinical Research,Fred Hutchinson Cancer Research Center, 2Departments of Medicine and Biochemistry,University of Washington

Chapters

  • 00:04Title
  • 00:44Preparing Cells and Sorting Using Flow Cytometry
  • 03:13DNA Extraction and Sequencing Library Preparation
  • 05:20Results: Late Replicating Sites in Budding Yeast
  • 06:14Conclusion

Summary

Automatic Translation

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我们描述了一种将流式细胞术和高通量测序相结合的技术来识别基因组的晚期复制区域。

Tags

G2-seqHigh-throughput SequencingDNA ReplicationCell CycleLate Replicating RegionsGenomeYeastFlow CytometryCell SortingDNA ContentG1SG2 Phases

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