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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Genetics
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JoVE Journal Genetics
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

English

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11,680 Views

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11:32 min

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May 24, 2017

DOI:

10.3791/55255-v

DOI:
10.3791/55255-v

•

11:32 min
May 24, 2017

•

5 Views
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1Stem Cell and Regenerative Biology, Genome Institute of Singapore,A*STAR, 2Computational and Systems Biology, Genome Institute of Singapore,A*STAR

Summary

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Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.

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RNA-RNA InteractionsSPLASHPsoralen CrosslinkingRNA InteractomeRNA GenomicsBiotinylated PsoralenUV CrosslinkingRNA IsolationGel ElectrophoresisSize Selection

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Aw, J. G. A., Shen, Y., Nagarajan, N., Wan, Y. Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen. J. Vis. Exp. (123), e55255, doi:10.3791/55255 (2017).

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