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Journal
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Biochemistry
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人视网膜解剖与 RPE-脉络膜的蛋白质组分析
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
Please note that all translations are automatically generated.
Click here for the English version.
人视网膜解剖与 RPE-脉络膜的蛋白质组分析
DOI:
10.3791/56203-v
•
06:54 min
•
November 12, 2017
•
Thiago Cabral*
1,2,7,8
,
Marcus A. Toral*
3,4
,
Gabriel Velez
4
,
James E. DiCarlo
2
,
Anuradha M. Gore
,
MaryAnn Mahajan
,
Stephen H. Tsang
2
,
Alexander G. Bassuk
6
,
Vinit B. Mahajan
9
1
Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons
,
Columbia University
,
2
Edward S. Harkness Eye Institute
,
New York-Presbyterian Hospital
,
3
Omics Laboratory, Byers Eye Institute, Department of Ophthalmology
,
Stanford University
,
4
Medical Scientist Training Program
,
University of Iowa
,
5
Department of Pediatrics
,
University of Iowa
,
6
Department of Neurology
,
University of Iowa
,
7
Department of Ophthalmology
,
Federal University of Sao Paulo (UNIFESP)
,
8
Department of Ophthalmology
,
Federal University of EspÍrito Santo (UFES)
,
9
Palo Alto Veterans Administration, Palo Alto, CA
Chapters
00:00
Title
01:15
Retina Dissection
03:00
RPE-Choroid Dissection
04:39
Scissors Dissection
05:15
Representative Results
06:11
Conclusions
Summary
Automatic Translation
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Automatic Translation
人类视网膜由功能和分子的不同区域组成, 包括黄斑、黄斑和外周视网膜。在这里, 我们描述了一个方法使用穿孔活检和手工清除组织层从人眼解剖和收集这些不同的视网膜区域的蛋白质组分析。
Tags
Retina
RPE-choroid
Proteomic Analysis
Fovea
Macula
Peripheral Retina
Punch Biopsy
Colibri Forceps
Westcott Scissors
Vitreous Gel
Sclera
One Dimensional STS Page
Article
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