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06:54 min
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November 12, 2017
DOI:
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons,Columbia University, 2Edward S. Harkness Eye Institute,New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology,Stanford University, 4Medical Scientist Training Program,University of Iowa, 5Department of Pediatrics,University of Iowa, 6Department of Neurology,University of Iowa, 7Department of Ophthalmology,Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology,Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
Summary
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人間の網膜は中心窩、黄斑の周辺部網膜など、分子の機能と異なる領域で構成されます。ここでは、細かく分析し、下流のプロテオーム解析のこれらの異なる網膜領域を収集パンチ生検と組織人間の目からレイヤーを手動で削除を使用して、メソッドについて述べる。
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Cite this Article
Cabral, T., Toral, M. A., Velez, G., DiCarlo, J. E., Gore, A. M., Mahajan, M., Tsang, S. H., Bassuk, A. G., Mahajan, V. B. Dissection of Human Retina and RPE-Choroid for Proteomic Analysis. J. Vis. Exp. (129), e56203, doi:10.3791/56203 (2017).
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