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大動脈リング共培養アッセイ:体外間葉系間質細胞の血管新生の可能性を評価するために便利なツール
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Developmental Biology
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JoVE Journal Developmental Biology
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
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大動脈リング共培養アッセイ:体外間葉系間質細胞の血管新生の可能性を評価するために便利なツール

日本語

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10,804 Views

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10:04 min

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September 18, 2017

DOI:

10.3791/56083-v

DOI:
10.3791/56083-v

•

10:04 min
September 18, 2017

•

14 Views
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1Create Fertility Centre, 2Department of Physiology,University of Toronto, 3Department of Obstetrics and Gynecology,University of Toronto, 4Department of Medical Sciences,University of Toronto, 5Department of Obstetrics and Gynecology,Women’s College Hospital

Summary

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ここでは、prelabelled の間葉系細胞がラット大動脈由来血管内皮ネットワークとの共培養して大動脈リング測定の新たな応用を提示します。この手法では、間葉系間質細胞 (MSCs) ホーミングと内皮細胞ネットワークとの統合の可視化、ネットワークのプロパティの定量化と MSC immunophenotypes と遺伝子発現の評価をことができます。

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Cite this Article

Iqbal, F., Gratch, Y. S., Szaraz, P., Librach, C. L. The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro. J. Vis. Exp. (127), e56083, doi:10.3791/56083 (2017).

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