Rivista
/
Biologia dello sviluppo
/
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

10,804 Views

•

10:04 min

•

September 18, 2017

DOI:

10.3791/56083-v

DOI:
10.3791/56083-v

•

10:04 min
September 18, 2017

•

14 Views
, , ,
1Create Fertility Centre, 2Department of Physiology,University of Toronto, 3Department of Obstetrics and Gynecology,University of Toronto, 4Department of Medical Sciences,University of Toronto, 5Department of Obstetrics and Gynecology,Women’s College Hospital

Summary

Automatically generated

Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.

Explore More Videos

Aortic RingCo-culture AssayAngiogenic PotentialMesenchymal Stromal CellsRegenerative TherapyVascular TargetingIschemic DiseasesFunctional AssayIn VitroBasal Membrane Extract (BME)Aorta IsolationIntercostal ArteriesAortic RingsEmbedding

Read Article

Cite this Article

Iqbal, F., Gratch, Y. S., Szaraz, P., Librach, C. L. The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro. J. Vis. Exp. (127), e56083, doi:10.3791/56083 (2017).

Download .ris file

Copy

Share Video

.

Copy