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Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

English

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9,819 Views

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14:55 min

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September 01, 2018

DOI:

10.3791/57684-v

DOI:
10.3791/57684-v

•

14:55 min
September 01, 2018

•

8 Views
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1Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences,Washington University School of Medicine

Summary

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This study presents a validated and optimized procedure for the isolation and culture of human endometrial stromal cells to conduct in vitro decidualization assay. Further, this study provides a detailed method to efficiently knockdown a specific gene using siRNAs in human endometrial stromal cells.

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Cite this Article

Michalski, S. A., Chadchan, S. B., Jungheim, E. S., Kommagani, R. Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization. J. Vis. Exp. (139), e57684, doi:10.3791/57684 (2018).

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