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The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
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JoVE Journal Gelişim Biyolojisi
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

10,804 Views

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10:04 min

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September 18, 2017

DOI:

10.3791/56083-v

DOI:
10.3791/56083-v

•

10:04 min
September 18, 2017

•

14 Views
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1Create Fertility Centre, 2Fizyoloji Bölümü,University of Toronto, 3Department of Obstetrics and Gynecology,University of Toronto, 4Department of Medical Sciences,University of Toronto, 5Department of Obstetrics and Gynecology,Women’s College Hospital

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Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.

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Iqbal, F., Gratch, Y. S., Szaraz, P., Librach, C. L. The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro. J. Vis. Exp. (127), e56083, doi:10.3791/56083 (2017).

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