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Birincil amniyotik sıvı ve membran hücreler Pluripotent Xeno-Alerjik koşullarında için yeniden programlama
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1Mitchell Cancer Institute,University of South Alabama, 2College of Medicine,University of South Alabama, 3Institute for Regenerative Medicine,University of Zurich, 4Department of Dermatology,University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM),University of Zurich – Irchel Campus
Chapters
- 00:05Title
- 00:50Isolation of Primary Mesenchymal Stem Cells from Human Amnion
- 02:31Harvesting Cultured Amniotic Fluid Stem Cells and Amniotic Membrane Stem Cells
- 03:47Transfection with Reprogramming Plasmids
- 05:35Picking Fully Reprogrammed Colonies for Clonial Expansion
- 06:43Clonal Expansion and Maturation of iPSCs
- 07:47Results: Morphological Progression of Cells During Reprogramming
- 08:43Conclusion
Bu iletişim kuralı birincil amniyotik sıvı ve membran Mezenkimal kök hücrelerin içine İndüklenmiş pluripotent kök hücreler tamamen kimyasal olarak tanımlanan koşullarda bir sigara entegre episomal yaklaşım kullanarak yeniden programlama açıklar. Yordamlar, ayıklama, kültür, yeniden programlama ve elde edilen İndüklenmiş pluripotent kök hücreler sıkı yöntemlerle karakterizasyonu ayrıntılı olarak açıklanmıştır.
Tags
Keyword Extraction:Amniotic FluidMembrane CellsPluripotencyXeno-free ConditionsInduced Pluripotent Stem CellsEpisomal ReprogrammingFetal Stem CellsDisease ModelingBasic ResearchLongitudinal StudiesChemically Defined ConditionsPediatric Cell-based TherapiesPlacentasAmnionRPMI-1640 MediumAFMC MediumAmniotic Fluid Stem CellsMembrane Stem CellsCell Detachment EnzymePBS
