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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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09:34 min
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November 27, 2017
DOI:
1Mitchell Cancer Institute,University of South Alabama, 2College of Medicine,University of South Alabama, 3Institute for Regenerative Medicine,University of Zurich, 4Department of Dermatology,University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM),University of Zurich – Irchel Campus
Summary
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This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
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Keyword Extraction:Amniotic FluidMembrane CellsPluripotencyXeno-free ConditionsInduced Pluripotent Stem CellsEpisomal ReprogrammingFetal Stem CellsDisease ModelingBasic ResearchLongitudinal StudiesChemically Defined ConditionsPediatric Cell-based TherapiesPlacentasAmnionRPMI-1640 MediumAFMC MediumAmniotic Fluid Stem CellsMembrane Stem CellsCell Detachment EnzymePBS
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Slamecka, J., Laurini, J., Shirley, T., Hoerstrup, S. P., Weber, B., Owen, L., McClellan, S. Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions. J. Vis. Exp. (129), e56003, doi:10.3791/56003 (2017).
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